宿主：兔
用途：WB,ICC, IHC
交叉反应性：人，小鼠，大鼠
分子量：～50KDa
推荐稀释比例：WB(1:1000),IF(1:100),IHC(1:50),ICC(1:50)
About this Antibody | |
Name | α-Tubulin Rabbit Polyclonal Antibody |
Category | Polyclonal antibody (pAb); Primary antibody |
Isotype | IgG |
Purification | Affinity purification |
About the Immunogen | |
Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to C-terminal α-Tubulin. |
Gene ID | 7277 |
SwissProt | P68366 |
Synonyms | ALS22;TUBA1;H2-ALPHA |
Category | Cell Cycle/Organism-specific biosystem |
Background | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular Tubulin subunits comprise the microtubule building block, with α/β-Tubulin heterodimers forming the Tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. |
注意事项：
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体 回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
2. 回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
储存条件：-20℃，有效期一年。
相关搜索：兔抗α-Tubulin抗体，α-Tubulin Rabbit Polyclonal Antibody